Figure 6.
Histology of representative mouse livers shows significant intracellular lipid accumulations in the high fat diet animal (a and c) as compared to the low fat diet animal (b and d). Marked micro and macrovacuolation in hepatocytes extends from the central vein mid-way to the portal triads in the high fat diet mouse (a, H&E stain). In contrast, the low fat diet mouse had no significant cytoplasmic vacuolation (i.e. lipid vacuoles) on H&E (b) and no positive staining for cytoplasmic lipid on ORO (d).