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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Protein Expr Purif. 2012 Jan 2;82(1):162–167. doi: 10.1016/j.pep.2011.12.008

Fig. 3.

Fig. 3

Expression and purification of BirA fusion proteins as followed by SDS-PAGE (12%). (A) The target protein was expressed as a thioredoxin fusion. Lane 1, cell lysate supernatant; Lane 2, cell lysate pellet (resuspended in 8 M urea); Lane 3, imidazole eluate (Ni-NTA resin purified protein); Lane 4, size-exclusion chromatography purified protein; Lane 5, protein standards. (B) The target protein was expressed as an MBP fusion. Lane 1, cell lysate supernatant; Lane 2, cell lysate pellet (resuspended in 8 M urea); Lane 3, affinity and size-exclusion chromatography purified fusion protein; Lane 4, protein standards.