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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Protein Expr Purif. 2012 Jan 2;82(1):162–167. doi: 10.1016/j.pep.2011.12.008

Fig. 4.

Fig. 4

Expression and purification of TEV protease as followed by SDS-PAGE (12%). Lane 1, cell lysate supernatant; Lane 2, cell lysate pellet (resuspended in 8 M urea); Lane 3, cell lysate supernatant after binding with Ni-NTA resin (unbound proteins); Lane 4, imidazole eluate (Ni-NTA resin purified protein); Lane 5, protein standards.