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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Protein Expr Purif. 2012 Jan 2;82(1):162–167. doi: 10.1016/j.pep.2011.12.008

Fig. 5.

Fig. 5

(A) Cleavage efficiency of the thioredoxin-BirA fusion protein with different amounts of TEV protease as followed by SDS–PAGE (12%). The reactions were allowed to proceed for 16 h at room temperature. Lane 1–6, 0, 2, 4, 6, 8, 10 μg of TEV protease was added to 1 mg of fusion protein, respectively; Lane 7, protein standards. (B) Separation of the target protein from the carrier thioredoxin by using a HisTrap HP column (5 ml). Lane 1–3, fractions that flowed through the column and which contain the target protein; Lane 4–5, factions that bound to the column and eluted with increasing concentration of imidazole, Lane 6, protein standards.