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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Protein Expr Purif. 2012 Jan 2;82(1):162–167. doi: 10.1016/j.pep.2011.12.008

Fig. 6.

Fig. 6

(A) thioredoxin and (B) MBP fusion cleavage and target protein purification as followed by SDS-PAGE (12%). Lane 1, corresponding fusion proteins purified by affinity and size-exclusion chromatography; Lane 2, reaction mixture after TEV protease cleavage; Lane 3, purified BirA; Lane 4, protein standards. The position of bands corresponding to the released BirA is indicated by an asterisk.