. Author manuscript; available in PMC: 2013 Mar 1.

Published in final edited form as: Protein Expr Purif. 2012 Jan 2;82(1):162–167. doi: 10.1016/j.pep.2011.12.008

Table 2.

Yields of fusion proteins and BirA from the two different constructs

	Thioredoxin-BirA (65.2%)^a	MBP-BirA (43.4%)
Purified fusion protein (mg/L)	45.9	79.2
Purified BirA (mg/L)	24.7 (29.9)^b	27.6 (34.4)

Note. In both cases, roughly 6.3 g wet cells were obtained from 1 liter of culture.

Mass percentage of the target protein in the corresponding fusion.

Theoretical yield calculated based on the amount of fusion protein and the corresponding mass percentage of the target protein in the two fusions.