Table 4.

TonB-dependent hemin and hemoglobin utilization in V. vulnificus

Strains	Iron sources
	FAC	Hemin	Hemoglobin
ΔvenB, ΔtonB1	++	++	++
ΔvenB, ΔttpC2	++	++	++
ΔvenB, ΔtonB2	++	++	++
ΔvenB, ΔtonB1, ΔtonB2	++	−	−
ΔvenB, ΔtonB1, ΔttpC2	++	−	−

⁺⁺Strong growth after 18 h

⁻no growth after 18 h. Indicated strains were mixed with CM9 media containing agar supplemented with 40 µM EDDA. After solidification, different iron sources (FAC, 1 mg/ml; hemin, 10 µM; and hemoglobin, 50 µM) were spotted on the plates and growth halos around the spots of the reporter strains were monitored after 18 h. FAC was used as a positive control