Biochemical analysis of mouse serums. Bars: Males (blue) and females (red). WT, wild-type; Mut, mutant (either hemizygote or heterozygote for Phex); KO, knockout (homozygote). Data are presented as mean ± SEM. Number of animals = 9-15 (4 weeks old) and 9-20 (12 weeks old) per group except 9-13 for 1,25(OH)2D. P-value < 0.05: a, compared to same-sex, same-age wild-type mice; b, Phex mutant mice; and c, Galnt3 knockout mice. a, b, and c, when both males and females of comparable genotypes had significant differences in the comparisons; (a), (b), and (c), when only one sex group had significant differences.