AJAP1 overexpression in glioma cells reduces cell proliferation and migration. A, overexpression of AJAP1 in D409MG glioma cells stably transfected with pEGFP-AJAP1 (open squares) reduces cell proliferation significantly compared to cells transfected with the empty vector, pEGFP-N1 (black squares). *P<0.05, D409MG+pEGFP-N1 compared to D409MG+pEGFP-AJAP1. B, overexpression of AJAP1 in D409MG glioma cells stably transfected with pEGFP-AJAP1 (hatched bar) reduces cell migration significantly compared to cells transfected with the empty vector, pEGFP-N1 (black bar). Experiments were done in triplicate. *P<0.05, D409MG+pEGFP-N1 at 4h compared to D409MG+pEGFP-AJAP1 at 4h, similar comparison at 6h and 8h.