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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Cancer Res. 2012 Jan 12;10(2):208–217. doi: 10.1158/1541-7786.MCR-10-0109

Figure 6.

Figure 6

Demethylation treatment in D54MG and U87MG restores AJAP1 expression. A, relative transcription level of AJAP1 in D54MG and U87MG before (lanes 1) and after treatment with AZA (lanes 2) and TSA (lanes 3), by Q-PCR. B, histogram showing relative levels of AJAP1 gene expression in D54MG and U87MG before (group 1) and after exposure to AZA (group 2) and TSA (group 3), (average of 3 blots). C, bisulfite sequencing of D54MG and U87MG before and after exposure to AZA and TSA. (Black circle >25% methylation; striped black circle = 10–25% methylation; gray circle <10% methylation; white circle = no methylation). D, migration results of D54MG after treatment with AZA and TSA show significant decrease in migration of cells (hatched bar) compared to untreated cells (black bars). SiRNA knockdown after AZA/TSA treatment partially restored migration ability (siRNA#1, gray bar; siRNA#2, diamond bar). Experiments were done in triplicate. *P<0.05.