Figure 2.
Activity of trypsin, chymotrypsin and elastase in intestinal luminal contents of sham animals using substrates specific for these enzymes (A). Western blot of intestinal luminal contents for the enzymes; values are mean±SEM (n=4) (B, C). In-vivo enzymatic activity of trypsin, chymotrypsin and elastase in whole intestine homogenates without removal of luminal contents (D). Western blot of whole intestine homogenates for the three enzymes; values are mean±SEM (n=4)/group ** P<0.001 compared to sham (E, F).