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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Shock. 2012 Mar;37(3):297–305. doi: 10.1097/SHK.0b013e318240b59b

Figure 4.

Figure 4

Representative micrograph of enzymatic activity on jejunal sections (4/animal) as assessed by in-situ zymography of substrates specific for trypsin, chymotrypsin and elastase (A). Enzymatic activity measured on the micrographs as mean fluorescent intensity of the fluorescent substrates, values are mean±SEM (n=4)/group (B). Enzyme penetration from the villi tip to the intestinal muscle measured on the micrographs (4/animal) as mean fluorescent intensity of the fluorescent substrates. Values are mean±SEM (n=4)/group, symbols used: Sham (circle), SAO15 (square), SAO30 (triangle) SAO15+TA (diamond), SAO30+TA (inverted triangle) (C). Western blot of trypsin, chymotrypsin and β-actin in intestine homogenates after removal of luminal contents (D). Relative density of trypsin and chymotrypsin and elastase with respect of β-actin. Values are mean±SEM (n=4)/group (E). *P <0.05, **P<0.001, ***P<0.0001 compared to sham. †P <0.05, ††P<0.001, †††P<0.0001 compared to 15 min SAO. ‡P <0.05, ‡‡P<0.001, ‡‡‡P<0.0001 compared to 30 min SAO.