Representative images of dose-dependent inhibition of intestinal epithelial wound healing with LPS 75 and 100 mcg/ml. Wounding assay. Images A, C, and E are 0 hr wounds and images B, D, and F are 8 hrs after wounding incubated with serum-free media and LPS treatment. G) LPS impairs wound healing in a dose-dependent fashion. Width healed (μm) was calculated by subtracting the area of the remaining wound at 8 hrs from the initial wound image at 0 hr. Values are means ± SD of two separate experiments. *, p < 0.001 vs untreated by ANOVA; †, p < 0.001 vs LPS 75 by ANOVA.