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. Author manuscript; available in PMC: 2013 Feb 14.
Published in final edited form as: Dev Cell. 2012 Jan 25;22(2):320–333. doi: 10.1016/j.devcel.2011.12.014

Figure 6.

Figure 6

PINK1 not localized to membranes does not interact with Parkin. (A) Mitochondrial Parkin does not recruit cytosolic PINK1 to mitochondria. HeLa cells were transfected with PINK1Δ110-YFP, mCherry-FKBP-Parkin and FRB-Fis1 and treated with or without rapalog for 2 h. The live cells were imaged by confocal microscopy. (B) Delayed Parkin translocation following the translocation of cytosolic PINK1-FKBP induced by heterodimerization. HeLa cells were transfected with PINK1Δ110-YFP-FKBP, mCherry-Parkin and FRB-Fis1 for 48 h, treated with rapalog and applied for confocal live cell imaging over 17 min. The first image was taken after 2 min of rapalog treatment, and subsequent images were taken after every 5 min. White bar, 10 μm. See also Figure S6.