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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Nat Chem Biol. 2012 Jan 22;8(3):262–269. doi: 10.1038/nchembio.771

Figure 2. Cellular stability of CK2α.

Figure 2

(a) Relative CK2α protein levels over time following cytoplasmic microinjection of REF52 cells with the HA-tagged semisynthetic proteins bearing different modifications using immunocytochemistry with anti-HA Ab. Data represent mean values ± s.d. (b) Relative CK2α protein levels following co-injection of β subunit with CK2α proteins. Data represent mean values ± s.d. (c) Relative CK2α protein levels following co-injection of proteasome inhibitor (1 µM MG132) with unmodified CK2α protein. Data represent mean values ± s.d. (d) Western blot for total CK2α protein in HeLa cells following treatment with Cdk inhibitors. Numbers indicate relative quantification normalized for loading control, mean values (n = 3) shown ± s.d. (e) Western blot for total CK2α protein in HeLa cells following treatment with nocodazole to arrest cells in mitosis. See Supplementary Fig. 8 for full gels corresponding to those in panels d, e.

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