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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: J Immunol. 2012 Jan 27;188(5):2437–2444. doi: 10.4049/jimmunol.1101070

Figure 6. miR-127 inhibited cytokine production and compliment component in IgG IC induced acute lung injury in vivo.

Figure 6

Mice received either PBS or miR-127 probes intranasally 2 h before onset of IgG IC model. At 4 h after IgG IC onset, mice were sacrificed and BALF or lung tissue was collected. A. Protein levels of IL-6, TNF-α, IL-1β, MIP-1β, MIP-2, MCP-1, and KC in BALF were measured with ELISA. B. Complement C5a protein in BALF was measured with ELISA. Values are presented as mean ± SEM. n = 3 - 6 in each group; *p < 0.05, **p < 0.01, ***p < 0.001 compared to IgG IC group. The experiments were repeated 3 times. C. Western blot was performed to determine STAT3 and phospho-STAT3 protein levels in lung tissues.