Fig. 2.

ADAM13 metalloproteinase activity is required for eye field formation and eye morphology. One dorsal-animal blastomere of 8-cell stage embryos was injected with the indicated MO (1.5 ng) and, as indicated, rescue mRNA encoding wild-type or the E/A mutant of ADAM13 (25 pg). (A) Embryos were allowed to develop to stage ~12.5 and then processed by in situ hybridization for pax6. A representative embryo of each injection is shown in the upper panels (the injected side is denoted with a red asterisk), and combined results summarized in graphs. (B) Embryos were scored for eye phenotype at stage ~35. **, p < 0.001; NS, not significant (p = 0.28). See Fig. 1A and Materials and Methods for phenotype scoring.