Fig. 3.

Effects of ADAM13 MO on eye development can be rescued by blocking EfnB signaling or by restoring canonical Wnt signaling. One dorsal-animal blastomere of 8-cell stage embryos was injected with the indicated MO (1.5 ng) and, as indicated, with 100 pg mRNA encoding EphB1ΔC, or full-length (FL) or deleted forms of Xdsh. (A) Embryos were cultured to stage ~12.5 and then processed by in situ hybridization for pax6. The injected side is denoted with a red asterisk. (B) Embryos were scored for eye phenotype at stage ~35. **, p < 0.001 in each case; NS, not significant (p = 0.17). See Fig. 1A and Materials and Methods for phenotype scoring.