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Precipitation of HCA with AMS and L3 from a cellular lysate. Images of SDS PAGE lanes showing (i) the total number of proteins remaining in 2.4 M AMS after centrifugation, (ii) the total number of proteins precipitated after the addition of 50 μM L3 ligand, (iii) the remaining HCA after the pellet of precipitated protein obtained from (ii) was washed with 1X PBS solution. The black box marks the bands corresponding to HCA.
