Skip to main content
. Author manuscript; available in PMC: 2013 Feb 17.
Published in final edited form as: ACS Chem Biol. 2011 Dec 12;7(2):260–268. doi: 10.1021/cb2003913

Figure 3.

Figure 3

Imaging Mb uptake by confocal microscopy. Fluorescence images were taken at 405 nm (cyan, mBBr-Mb) and 543 nm (red, the lipophilic membrane dye FM 4–64). The merged images show the distribution of both signals. The scale bar corresponds to 4 μm. M. trichosporium OB3b cells incubated with mBBr-Cu-Mb and FM 4–64 (top) or mBBr-Mb and FM 4–64 (middle) exhibit primarily cytoplasmic localization of the compound. The background fluorescence of M. trichosporium OB3b cells, which presumably includes the intrinsic fluorescence of internally produced Mb (bottom, also in the presence of FM 4–64) is also detectable, but is of a much lower magnitude.