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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Hepatology. 2012 Mar;55(3):931–940. doi: 10.1002/hep.24766

Figure 7. KO mice have decreased expression of major alcohol metabolizing pathways.

Figure 7

A. ADH and ALDH activity assays (n = 5/group). B. Real-time PCR analysis of Adh1 and Aldh2 genes in the liver (n = 5–6/group). C. Western blot analysis of ADH1 and ALDH2 proteins in the liver. GAPDH is shown as internal loading control. D. Densitometric analysis of western blot shown in Figure 7C with normalization to GAPDH. E. Representative western blot analysis showing Cyp2E1 protein levels in hepatic microsomal preparation. F. Densitometric analysis of microsomal Cyp2E1 levels.