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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: Clin Cancer Res. 2012 Jan 6;18(4):1039–1050. doi: 10.1158/1078-0432.CCR-11-1429

Figure 2. Linear relationship between CD20 expression versus rituximab-associated CMC was observed only in single cell clones from RSCL. A threshold of surface CD20 density appeared to be required for efficient cytotoxicity.

Figure 2

Single cell clones of parentalRajiand the RRCL, Raji2R, were obtained by limiting dilution. ImageStream analysis of approximately 5,000 cells per clone showed: (A) Mean surface CD20 density in each clone was calculated as described earlier. Rituximab-associated CMC in different clones was determined by 51Cr release assay. (B) Surface CD20 expression was plotted against rituximab-associated CMC to determine their correlation. (C, D) Surface CD55 and CD59 expressions of individual clones was determined by ImageStream.