Figure 5.

The FOXO3a effectors, BNIP3 and MurF-1, are significantly upregulated in POH and peaked in heart failure. (a) Western blot analysis of LV tissue lysates from CTL, Week 1 POH (POH, W1), Week 3 POH (POH, W3) and HF samples. JNK and AKT activities as well as Bax to Bcl-2 ratio are significantly increased 1 week after POH and remained to be elevated up to heart failure, ^*P<0.05 versus CTL. The phospho-FOXO3a at Ser253 and the expression of BNIP3 and MurF-1 were significantly increased 3 weeks after pressure overload and peaked in heart failure, ^#P<0.05 versus CTL and POH, W1, ^$P<0.05 versus all the three groups. (b) Western blot analysis of LV tissue lysates from normal and failing human hearts. JNK and AKT activities as well as phospho-FOXO3a Ser253 and phospho-Bcl-2 and BNIP3 expression are robustly increased in heart failure, ^*P<0.05 versus CTL