Table 1. Genetic interactions of L, hyrax, and Tor.
| Cross No. | Parent 1 | Parent 2 | F1 genotype | % flies with NOGa eye phenotype | % NOG in both eyes | % notch w/o OG | Molecular function |
|---|---|---|---|---|---|---|---|
| 1 | w1118 | w1118 | w1118 | 0 | 0 | 0 | |
| 2 | Lsi/si | w1118 | Lsi/+ | 0 | 0 | 100 | L (lobe) is the fly homolog of mammalian AKT1S1 (also called PRAS40) |
| 3 | hyxEY6898/+ | Lsi/+hyx EY6898/+ | 6 | 0 | 11 | Hyx (hyrax) is the fly homolog of CDC73/ HRPT2 | |
| 4 | hyxEY6898/+ | w1118 | hyxEY6898/+ | 0 | 0 | 0 | |
| 5 | TorMB07988/MB07988 | TorMB07988/MB07988 | TorMB07988/MB07988 | 0 | 0 | 0 | Tor is a key cellular kinase involved in the regulation of cell growth and nutritional homeostasis |
| 6 | w1118 | TorMB07988/+ | 0 | 0 | 0 | ||
| 7 | Lsi/+ | Lsi/TorMB07988 | 6 | 0 | 2 | ||
| 8 | Lsi/+hyx EY6898/+ | Lsi/TorMB07988; hyx EY6898/+ | 40*** | 7 | 3 | ||
| 9 | hyxEY6898/+ | TorMB07988/+ hyxEY6898/+ | 0 | 0 | 0 | ||
| 10 | TorK17004/+ | Lsi/+ hyx EY6898/+ | Lsi/TorK17004; hyxEY6898/+ | 45*** | 8 | 7 | |
| 11 | Lsi/TorK17004 | 10 | <1 | 9 | |||
| 12 | hyxEY6898/+ | TorK17004/+ hyxEY6898/+ | 0 | 0 | 0 |
a
NOG: notch and overgrowth (OG), with notches and variably sized dysplastic overgrowths in the ventral eye fields.
***P<0.0001 compared with w1118n, and all double and single heterozygote mutants