Figure 1.

Enhanced in vivo iTreg induction and decreased incidence of IBD in the absence of Bim. Tconv were sorted from the spleen and lymph nodes of C57BL/6 (WT) or Bim^−/− mice and injected i.v. into the tail veins of Rag1^−/− recipients (0.4 × 10⁶ cells per mouse). Four weeks post-transfer, spleen, MLN and IEL/LPL (intestine) were analyzed by flow cytometry. Percentage and number of CD4⁺ (a and b) and CD4⁺Foxp3⁺ (c and d) cells. Data are the mean±S.E. of two to three individual experiments with five to seven mice per group (a−d), and statistical analysis performed using standard unpaired t-test (^*P<0.05, ^**P<0.01). (e) Representative dot plots of CD4⁺Foxp3⁺ iTregs. (f) Percentage of BrdU⁺ Tconv (CD4⁺Foxp3⁻, left) and iTregs (CD4⁺Foxp3⁺, right). Data are the mean±S.E. of two independent experiments with four mice per group, and statistical analysis performed using standard unpaired t-test (^*P<0.05, ^**P<0.01). (g) Incidence of IBD in Tconv recipients and statistical summary (2 × 2 χ²-analysis)