Figure 3.

Intrinsic vulnerability of Bim, Bcl-2 and Bcl-xl balance in iTregs. (a and b) iTregs and activated Tconv were prepared as in Figure 2. mRNA was isolated from either freshly sorted iTregs (Foxp3⁺) and activated Tconv (Foxp3⁻), before (pre) or after an additional 48 h of culture in the absence of IL-2 (post). Expression levels of genes indicated was assessed by qPCR. Data shown are the mean±S.E. from five independent experiments with statistical analysis performed using standard paired t-test (^*P<0.05, ^**P<0.01, ^***P<0.005). (c) Intracellular Bcl-2 of freshly sorted iTregs (Foxp3⁺) and activated Tconv (Foxp3⁻) before (pre) or after an additional 48 h of culture in the absence of IL-2 (post). Data represent two independent experiments