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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Mol Immunol. 2012 Feb 1;50(3):125–133. doi: 10.1016/j.molimm.2011.12.013

Fig. 2. Numbers of IL-17+ cells are greatly increased in B6 mice immunized with IRBP1-20 in CFA, but not IRBP1-20 in IFA.

Fig. 2

(A) B6 mice were immunized with the uveitogenic peptide IRBP1-20 in IFA or CFA, then splenic T cells were stimulated for 48 h with either IRBP1-20 (left panels) or anti-CD3 antibody (right panels) and the activated T cells stained with PE-conjugated anti-IFN-γ antibodies and FITC-conjugated anti-IL-17 antibodies, followed by FACS analysis. (B) IL-17 levels in the culture supernatants at 48 h assessed by ELISA. (C) Frequencies of IFN-γ+ and IL-17+ IRBP1-20 -specific T cells determined by LDA. The results shown are representative of those from >5 experiments. The differences between the CFA and IFA groups were highly significant (P ≤ 0.01) in all three assays.