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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Mol Immunol. 2012 Feb 1;50(3):125–133. doi: 10.1016/j.molimm.2011.12.013

Fig. 3. IFA containing synthetic TLR ligands enhances the in vivo priming of IL-17+ T cells in B6 mice.

Fig. 3

(A) Determination of IL-17+ cells by intracellular staining. Groups of mice (n=4) were immunized with IRBP1-20/CFA, IRBP1-20/IFA, or IRBP1-20/IFA containing TLR2 and TLR4 ligands or TLR3 and TLR9 ligands. The in vivo primed T cells were then stimulated with IRBP1-20 or anti-CD3 antibodies in the presence of syngeneic APCs for 5 days under Th17 polarized conditions and activated T cells stained with PE-conjugated anti-IFN-γ antibodies and FITC-conjugated anti-IL-17 antibodies, followed by FACS analysis. (B) IL-17 levels in the culture supernatants assessed by ELISA 48H after stimulation. (C) LDA results showing the number of IFN-γ (Th1) or IL-17+ (Th17) T cells among 100,000 in vivo primed responder T cells. The results shown are representative of those in more than three experiments.