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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Mol Immunol. 2012 Feb 1;50(3):125–133. doi: 10.1016/j.molimm.2011.12.013

Fig. 5. Immunized TCR-δ−/− mice generate fewer IL-17+ autoreactive T cells than immunized B6 mice.

Fig. 5

(A) B6 and TCR-δ−/− mice were immunized with IRBP1-20/CFA, then, 13 days later, splenic T cells were enriched and assessed for Th1 and Th17 IRBP1-20-specific T cells by LDA. (B) Splenic T cells from immunized B6 and TCR-δ−/− mice were stimulated with IRBP1-20 and APCs under Th17 polarizing conditions for 5 days, then the activated T cells were separated, fixed, and stained with anti-IFN-γ, anti-IL-17, and anti-αβ TCR antibodies.