Fig. 7. Treatment of B6 mice with a single dose of TLR ligand-activated γδ T cells rendered γδT cells acquired enhanced effect promoting the generation of IL-17⁺ autoreactive T cells in vivo.

(A) Splenic T cells from B6 mice immunized with IRBP_1-20/IFA, IRBP_1-20/CFA, or IRBP_1-20/IFA containing TLRL2 and TLR4 ligands or TLRL3 and TLR9 ligands were examined for γδTCR⁺ T cells 14 days after immunization. (B) γδ T cells freshly isolated from B6 mice immunized with CFA or IFA containing TLR ligands 2 and TLR4 produce increased amounts of IL-17. (C) γδ T cells were isolated from B6 mice immunized with IRBP_1-20/CFA, IRBP_1-20/IFA, or IRBP_1-20/IFA containing TLRL2 and TLR4 ligands or TLRL3 and TLR9 ligands and 100,000 isolated γδ T cells from each group were injected into four different groups of TCR-δ^−/− recipient mice, then all the recipient mice were immunized with IRBP_1-20/CFA, and IFN-γ⁺ and IL-17⁺ IRBP_1-20 -specific responder T cells were assessed by LDA. The results shown are representative of those from three experiments.