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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Methods. 2011 Oct 19;56(3):408–417. doi: 10.1016/j.ymeth.2011.10.004

Figure 3.

Figure 3

Example growth profiles for biological triplicate conditions. A) The conditions being examined did not significantly affect the growth rate; however, the final cell densities were significantly different. In this case, obtaining gene expression analysis data from one time point, late in culture (around 90 hours, indicated by the vertical line) would not have high noise in the data due to a growth effect. B) The conditions being examined significantly affect the growth rate. In this case, the stress was applied at 40 hours. Sampling throughout the culture's growth (before and after the stress) would be desirable, such that gene expression changes due to growth effect could be identified. The total RNA sample times are indicated by the vertical lines.