Fig. 5.

Western blot analysis of the mitochondrial proteins UCP-2 and PGC1α as well as the Ca²⁺ regulatory proteins SERCA2a, Na⁺-Ca²⁺ exchanger (NCX) and phospholamban in myocardium from LF and HF-fed FVB and IGF-1 mice. A: Representative gel blots of UCP-2, PGC1α, SERCA2a, Na⁺-Ca²⁺ exchanger, phospholamban and GAPDH (loading control) using specific antibodies; B: UCP-2; C: PGC1α; D: SERCA2a; E: Na⁺-Ca²⁺ exchanger; and F: Phospholamban. All proteins were normalized to the loading control GAPDH. Mean ± SEM, n = 8–9 mice per group, *p <0.05 vs. FVB-LF group, #p < 0.05 vs. FVB-HF group.