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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Hypertension. 2012 Jan 23;59(3):680–693. doi: 10.1161/HYPERTENSIONAHA.111.181867

Fig. 6.

Fig. 6

Western blot analysis of pan and phosphorylated insulin receptor β or IGF-1 receptor β in cardiomyocytes from LF and HF-fed FVB and IGF-1 mice. A: Insulin receptor β expression; B: Basal and insulin/IGF-1-stimulated (at 100 nM for 15 min) tyrosine phosphorylation of insulin receptor β; C: IGF-1 receptor β expression; D: Basal and insulin/IGF-1-stimulated (at 100 nM for 15 min) tyrosine phosphorylation of IGF-1 receptor β; E: Tyrosine phosphorylation of IRS (Tyr1146) normalized to pan IRS; and F: Serine phosphorylation of IRS (Ser307) normalized to pan IRS; Insets: Representative gel blots of pan and phosphorylated insulin receptor β, IGF receptor β, and IRS using respective specific antibodies. Protein expressions were normalized to that of FVB-LF group for immunoprecipitation studies. pY denotes anti-phosphotyrosine antibody; IP and IB represent immunoprecipitation and immunoblot, respectively; Mean ± SEM, n = 5–7 mice per group, *p <0.05 vs. un-stimulated FVB-LF group, †p < 0.05 vs. insulin-stimulated FVB-LF group, #p < 0.05 vs. respective FVB-HF group.