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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: Int J Cancer. 2011 Oct 20;131(2):377–386. doi: 10.1002/ijc.26379

Figure 1.

Figure 1

CHI3L1 is upregulated in mammary tumor-bearing mice. (A) Serum was collected from normal and 5-week tumor-bearing mice and analyzed for CHI3L1 expression by ELISA. (B) CHI3L1 expression in DA-3 mammary tumor cells was localized by immunofluorescence using confocal microscopy, scale bar = 20 μM. (C) Purified total RNA from splenocytes of normal and 5-week DA-3 tumor bearers was used to measure expression of CHI3L1 by qRT-PCR. (D–E) Purified myeloid derived cells (D) or macrophages (E) were cultured overnight and cell-free supernatants were analyzed for CHI3L1 expression by ELISA. (F) Purified macrophages from normal and 5-week DA-3 tumor bearers were examined by immunolocalization of CHI3L1 expression and visualized by confocal microscopy, scale bar = 20 μM. For all experiments, N = 8 mice/group, *p<0.0005, Student's t-test. 159×144mm (300 × 300 DPI)