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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: Cancer Res. 2011 Dec 20;72(4):949–957. doi: 10.1158/0008-5472.CAN-11-2371

Figure 2. HIF1α gene targets are transcriptionally upregulated in IMEC-Myc cells.

Figure 2

A) IMECs were transfected with non-targeting siRNA or siRNAs (1 or 2) targeting HIF1A or c-myc. RNA was isolated 48 hrs post-transfection. Real-time RT-PCR was performed using primers specific to HIF1A, c-myc, and GAPDH in triplicate. Average RNA levels were normalized to GAPDH and then normalized to their respective normoxic vectors. B) Real-time RT-PCR was performed using primers specific to ALDOA, HK2, eNOS, BGN, and GAPDH in triplicate. Average RNA levels were normalized to GAPDH and then normalized to their respective normoxic vectors. Error bars reflect the standard deviation.