A) Western blot analyses of IMEC cells were performed with equivalent amounts of protein using anti-VHL, anti-CUL2, anti-RBX1, anti-Elongin B, anti-Elongin C, and anti-β-tubulin antibodies. B) Real-time RT-PCR was performed using RNA harvested from IMECs and primers specific to VHL, CUL2, RBX1, Elongin B (TCEB2), Elongin C (TCEB1), and GAPDH in triplicate. Average RNA levels are normalized to GAPDH and then normalized to their respective normoxic vectors. C) Western blot analyses of myc−/− rat fibroblasts (+/− reconstituted mouse c-Myc) were performed as described above. Error bars reflect the standard deviation.