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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: Cancer Res. 2011 Dec 20;72(4):949–957. doi: 10.1158/0008-5472.CAN-11-2371

Figure 5. Myc enhances post-transcriptional accumulation of VHL complex components under both normoxia and hypoxia.

Figure 5

A) Western blot analyses of IMEC cells were performed with equivalent amounts of protein using anti-VHL, anti-CUL2, anti-RBX1, anti-Elongin B, anti-Elongin C, and anti-β-tubulin antibodies. B) Real-time RT-PCR was performed using RNA harvested from IMECs and primers specific to VHL, CUL2, RBX1, Elongin B (TCEB2), Elongin C (TCEB1), and GAPDH in triplicate. Average RNA levels are normalized to GAPDH and then normalized to their respective normoxic vectors. C) Western blot analyses of myc−/− rat fibroblasts (+/− reconstituted mouse c-Myc) were performed as described above. Error bars reflect the standard deviation.