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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Tuberculosis (Edinb). 2012 Jan 4;92(2):139–147. doi: 10.1016/j.tube.2011.11.010

Table 1.

Purification of M. tuberculosis Rv0535 from E. coli BL21 (DE3)

Purification Step Total protein (mg) Total Activity (nmoles hr−1) Specific Activity a (nmoles mg−1 hr−1) Purification (fold) Yield (%)
Crude cell extract 180 89,000 480 1 100
HisTrap HP 0.20 4,400 22,000 46 5
a

Activity determined with 100 μM MTA plus 50 mM phosphate