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Substrate specificity of Rv0535
| Nucleosides (100 μM) | Enzyme Activity (nmoles mg−1 hr−1)a | Relative activity (%) |
|---|---|---|
| Purines | ||
| 5′-Methylthioadenosine | 99,000 ± 3,000 | 100 |
| Adenosine | 2,100 ± 200 | 2.1 |
| S-Adenosyl-L-homocysteine | 800 ± 100 | 0.81 |
| 2′-Deoxyadenosine | -- | -- |
| Inosine | -- | -- |
| Guanosine | -- | -- |
| Xanthosine | -- | -- |
| 5′-Methylthioinosine | -- | -- |
| Pyrimidines | ||
| Cytidine | -- | -- |
| Thymidine | -- | -- |
| Uridine | -- | -- |
values represent mean ± standard deviation for 3 determinations
-- less than 700 nmoles mg−1 hr−1
