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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Alcohol Clin Exp Res. 2011 Oct 21;36(4):641–653. doi: 10.1111/j.1530-0277.2011.01660.x

Figure 7. Ethanol feeding differentially affects immune responses in male versus female mice in the setting of hepatocarcinogenesis.

Figure 7

A) Quantitative real time PCR (qRT-PCR) analysis of T-bet, GATA3, and SMAD3 mRNA expression in total hepatic mRNA isolated from control (C) and pair-matched animals maintained on ethanol (E), DEN-initiated (D), or DEN-initiated followed by ethanol feeding (D+E) at 48-weeks. *p<0.05 versus C, #p<0.05 versus respective male or female E mice, §p<0.05 male versus female within same treatment group/time points. B) Custom BioPlex assay was used to determine cytokine expression in pooled serum samples from control male (left) and female (right) mice (C), and pair matched animals maintained on ethanol (E), DEN-initiated (D), or DEN-initiated followed by ethanol feeding (D+E) at 48 weeks. Minimum n = 8 independent samples per group.