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Inhibition of miR-20a increased Fas expression and sensitivity to FasL. A, LM7 cells were transfected with anti–miR-20a or control for 72 hours. Fas expression and β-actin were then measured by Western blotting. B,C, LM7 control and anti–miR-20a transfected cells were incubated with FasL or medium for 24 hours. Cell survival was measured by MTT assay (B) (*, P < 0.05). Apoptotic cells (average from 5 high-power microscope fields) were quantified by TUNEL assay (C).
