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. Author manuscript; available in PMC: 2013 Feb 24.
Published in final edited form as: Immunity. 2012 Feb 2;36(2):276–287. doi: 10.1016/j.immuni.2011.12.011

Figure 2. TLR5-Expressing Dendritic Cells are Required for Flagellin-Mediated IL-22 Expression.

Figure 2

(A – B) Mixed bone marrow (BM) chimeric mice in which the hematopoietic compartment is composed of equal ratios of Tlr5−/−, CD11c.DTR or wild-type (WT) BM cells, in combinations indicated on the horizontal axes, were depleted of WT dendritic cells by intraperitoneal injection of diphtheria toxin. Messenger RNA (mRNA) expression in the small intestine (SI) lamina propria (A) and serum protein amounts (B) of IL-22 in response to flagellin (Flg) administration were assessed 3 hours after injection (n = 2 – 4 mice per group). Control (Ctrl) groups received PBS.

(C) Bone marrow chimeric mice, generated by transferring bone marrow from CD11c.DTR mice into irradiated WT mice, were depleted of DCs using diptheria toxin as described in the Methods section. RegIIIγ mRNA expression in the small intestine was assessed 24 hours following intravenous injection of flagellin. (n = 3 mice per group). Error bars denote ± s.e.m. Data are pooled from two independent experiments.