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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Arthritis Rheum. 2011 Oct 27;64(4):1182–1192. doi: 10.1002/art.33444

Figure 2. ULK1, Beclin1 and LC3 expression is reduced in response to mechanical injury.

Figure 2

Full-thickness cartilage explants (n=24) were subjected to mechanical injury or cultured without injury to evaluate the effect of mechanical injury on the autophagy pathway. A, Total protein from explants subjected to mechanical injury or cultured without injured at 24, 48 and 96 hours was analyzed by Western blotting using anti-LC3 and β-actin. B, Safranin O staining and immunohistochemical analysis of ULK1, Beclin1 and LC3 from explants subjected to mechanical injury or cultured without injury at 48 hours. Original magnification x40. C–E, Quantitative analysis of ULK1, Beclin1 and LC3 positive cells in superficial zone (SZ), mid zone (MZ) and deep zone (DZ). Values represent mean ± SD of four separate experiments each in duplicate, * = P < 0.05.