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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Arthritis Rheum. 2011 Oct 27;64(4):1182–1192. doi: 10.1002/art.33444

Figure 3. Rapamycin does not affect cell viability and increases expression of autophagy regulators.

Figure 3

Full-thickness cartilage explants and bovine chondrocytes were employed to study the potential effect of rapamycin on cell viability by Live-Dead assay. Autophagy activation was assessed by immunohistochemistry. A, Results of Live/Dead viability assay at 48 hours. B, LC3 expression in explants in control conditions and after rapamycin treatment (Rapa; 1, 5 and 10 μM) at 48 hours. Original magnification X 40.