Table 3.

Back-calculated concentrations from calibration curves run in duplicate on four occasions in human plasma

Table 3A Irinotecan
Nominal (ng/mL)	GMa (ng/mL)	S.D. (ng/mL)	DEV(%)	R.S.D.(%)	n
5	5.10	0.085	1.99	1.67	8
10	9.67	0.294	−3.28	3.04	8
20	19.6	0.598	−1.81	3.04	8
50	49.9	0.906	−0.16	1.82	8
100	101.4	3.00	1.46	2.96	8
250	254.4	4.29	1.76	1.69	8
500	508.8	15.2	1.76	2.98	8
1000	982.8	22.9	−1.72	2.33	8
Table 3B SN38
Nominal (ng/mL)	GMa (ng/mL)	S.D. (ng/mL)	DEV(%)	R.S.D.(%)	n
0.5	0.51	0.013	2.53	2.63	8
1.0	0.97	0.021	−2.60	2.14	8
2.0	1.93	0.034	−3.33	1.77	8
5.0	4.79	0.143	−4.24	3.00	8
10	9.88	0.269	−1.22	2.72	8
25	25.4	1.18	1.76	4.64	8
50	51.6	1.84	3.14	3.57	8
100	103.9	3.18	3.97	3.06	8
Table 3C SN38-G
Nominal (ng/mL)	GMa (ng/mL)	S.D. (ng/mL)	DEV(%)	R.S.D.(%)	n
0.5	0.52	0.025	3.40	4.91	8
1.0	0.95	0.062	−5.11	6.56	8
2.0	1.97	0.114	−1.68	5.82	8
5.0	4.73	0.160	−5.45	3.40	8
10	10.02	0.528	−0.21	5.27	8
25	25.9	0.627	3.57	2.42	8
50	51.4	1.46	2.69	2.84	8
100	102.4	3.35	2.38	3.27	8

^aAbbreviations: GM, grand mean; S.D., standard deviation; DEV, percent deviation from nominal value; R.S.D., relative standard deviation; n, total number of replicate observations within the validation runs.