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. Author manuscript; available in PMC: 2013 Feb 14.
Published in final edited form as: Dev Cell. 2012 Jan 19;22(2):446–458. doi: 10.1016/j.devcel.2011.11.021

Figure 1. Nup210 expression is induced during myogenic differentiation.

Figure 1

(A) mRNA and protein levels of Nup210 during C2C12 differentiation were analyzed by qPCR and western blot respectively (n=3). Nup210 mRNA levels in differentiating cells were normalized to the expression of dividing cells (Day 0). Myosin Heavy Chain (MHC) was used as a differentiation marker and Histone H3 (His H3) as loading control. Data are presented as average values ± SD. (B) Nup210 mRNA and protein levels in dividing myoblasts (M), differentiated C2C12 cells (D), myotubes (T) and quiescent myoblasts (Q) were analyzed by semi-quantitative PCR and western blot respectively. (C) Immunofluorescence against Nup210 and Pom121 in dividing and differentiated (Day 4) C2C12 cells. MHC was used as a maker for differentiated myotubes and nuclei were stained with Hoechst. (D) Higher magnification of immunofluorescence against Nup210 and Pom121. Arrows indicate quiescent myoblasts. See also Figure S1.

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