Figure 2. Nup210 is required for myoblast differentiation.

(A) C2C12 cells were infected with lentivirus carrying control or Nup210-specific shRNAs. Infected cells were selected and induced to differentiate. Differentiated myotubes were stained against MHC at 48, 72, and 96 hours after differentiation. Nuclei were stained with Hoechst. (B) The percentage of nuclei in multinucleated cells (>2 nuclei) to the total number of nuclei in the field (Fusion Index) in panel (A) was quantified. Values represent the average of 4 different independent experiments ± SD. (C) Quantification of MHC positive cells shown in panel (A). Percentage of cells containing 1 (mononuclated), 2 (binucleated), or more nuclei (multinucleated) were determined at different times of differentiation. Data are presented as average values of 3 independent experiments. (D) C2C12 cell lines carrying control or Nup210 shRNAs were transfected with GFP or an RNAi resistant Nup210-GFP. Cells were reselected, induced to differentiate and stained against MHC on Day 4. Inlets show a higher magnification of GFP and Nup210-GFP expressing cells. (E) Fusion Index for the experiment in panel (E) was quantified as described. See also Figure S2.