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. Author manuscript; available in PMC: 2013 Feb 14.
Published in final edited form as: Dev Cell. 2012 Jan 19;22(2):446–458. doi: 10.1016/j.devcel.2011.11.021

Figure 5. Ectopic expression of Nup210 in myoblasts increases the expression levels of differentiation genes and accelerates myotube formation.

Figure 5

(A) The expression levels of Asb2, Clic5, GDF5, Neu2 and Ndrg2 in GFP and Nup210-GFP expressing myoblasts were analyzed by qPCR. mRNA levels for each gene was normalized to the levels of GFP expressing cells. Values represent average ± SD of 3 different independent experiments. (B) GFP and Nup210-GFP C2C12 expressing myoblasts were induced to differentiate. Immunofluorescence against MHC was performed at 48, 72, and 96 hours post differentiation. (C) Fusion Index (percentage of nuclei in multinucleated cells (>2 nuclei) to the total number of nuclei in the field) for experiment in panel B. Values represent the average of 4 different independent experiments ± SD. (D) Percentage of cells containing 1–4, 5–10, 11–20 or >20 nuclei in panel B were determined at different times of differentiation. Data are presented as average values of 3 independent experiments. (E) C2C12 myoblasts ectopically expressing Nup210-GFP were infected with lentivirus carrying control, Clic5, GDF5 or Neu2 shRNAs and induced to differentiate. Immunofluorescence against MHC was performed at 24, 48 and 72h post differentiation. GFP overlay shows the expression of Nup210-GFP. See also Figure S6.