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. Author manuscript; available in PMC: 2013 Feb 14.
Published in final edited form as: Dev Cell. 2012 Jan 19;22(2):446–458. doi: 10.1016/j.devcel.2011.11.021

Figure 6. Nup210 is essential for neuronal differentiation.

Figure 6

(A) Embryonic stem (ES) cells were induced to differentiate into neuroprogenitor (NP) cells and stained for Nup210. Oct4 and Nestin were used as specific markers for ES and NP cells respectively. Hoechst was used to stain nuclei. (B) ES cells were infected with lentivirus carrying control or Nup210 shRNAs and induced to differentiate into NP cells. Differentiated cells were stained against Nup210 and Nestin. Hoechst was used to stain nuclei. (C) Differentiated NP cells were infected with lentivirus carrying control or Nup210 shRNA and stained against Nup210 and Nestin. (D) Control or Nup210 knockdown NP cells were stained with Nestin and Cleaved Caspase-3 (Caspase 3) antibodies. (E) Schematic model of Nup210 regulation of cell differentiation. In undifferentiated myoblast the expression of Nup210 is repressed. Early differentiation signals activate Nup210 gene expression. In myoblast, Nup210 induction is likely carried out by Myogenin/MyoD binding to its promoter E-boxes. Nup210 protein is then recruited to the NPC where it regulates the expression of genes required for myogenic and neuronal differentiation. Prevention of Nup210 addition to the NPC by shRNAs prevents the activation of Nup210 regulated genes and leads to the death of the differentiation-committed cell by apoptosis.