Figure 3. Mechanism of action (MOA) and inhibition studies of compound 1 with HePTP and HePTP mutants.
(a) Progress curves of HePTP activity in the presence of different doses of compound 1. No time-dependent inhibition was observed as demonstrated by the linear progress curves of the HePTP phosphatase reaction. (b) Eadie-Hofstee plot of the Michaelis-Menten kinetic studies with compound 1. (c) Dose-response curves for compound 1 with either wt HePTP, HePTP-DM, or HePTP H237A.