Figure 6. Compound 1 causes sustained hyperactivation of ERK1/2 and p38 in human T cells.
ERK1/2 and p38 phosphorylation in Jurkat TAg T cells, preincubated with vehicle (DMSO, 0.2%) or compound 1 (4 μM), at various time points after TCR stimulation. Phospho-protein bands were normalized to total protein levels and then to GAPDH. Statistical significance was determined by one-way ANOVA with post hoc Tukey’s test (within group (compound 1- or DMSO-treated samples, respectively), **p<0.01), or Student’s t-test (between groups (between compound 1- and DMSO-treated samples), #p< 0.05, ##p< 0.01). Representative blots are shown from 4 independent experiments.